Visualizing enzyme intermediates using fast diffraction and reaction trapping methods: isocitrate dehydrogenase.
نویسنده
چکیده
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منابع مشابه
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase.
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate interm...
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متن کاملCombining X-ray crystallography and single-crystal spectroscopy to probe enzyme mechanisms.
The combination of X-ray crystallography and rapid cryo-trapping methods has enabled the visualization of catalytic intermediates in a variety of enzyme systems. However, the resolution of the X-ray experiment is not always sufficient to precisely place the structure on the reaction pathway. In addition, many trapped intermediates are X-ray-sensitive and can decay during diffraction data collec...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 27 2 شماره
صفحات -
تاریخ انتشار 1999